abstract：:mRNAs extracted from human pheochromocytoma were translated in vitro in a lysate of a rabbit reticulocytes. Two enzymes of the biosynthetic pathway of the catecholamines, tyrosine-hydroxylase (TH) and dopamine-beta-hydroxylase (DBH), were characterized as translation products after immunoprecipitation by specific anti...

journal_title：Biochimie

authors： Benlot C,Antreassian J,Henry JP,Legrand JC,Gros F,Thibault J

更新日期：1985-06-01 00:00:00

abstract：:We describe PGtrans, a new and freely available protein sequence databank (2625 sequences, 554198 amino-acids). This data bank is routinely produced by automatic computer translation of the nucleotide sequence library GenBank. The information needed for the translation process (transcriptional orientation, location of...

journal_title：Biochimie

authors： Claverie JM,Sauvaget I,Bougueleret L

更新日期：1985-05-01 00:00:00

abstract：:A set of sequences can be defined by their common subsequences, and the length of these is a measure of the overall resemblance of the set. Each subsequence corresponds to a succession of symbols embedded in every sequence, following the same order but not necessarily contiguous. Determining the longest common subsequ...

journal_title：Biochimie

authors： Saurin W,Marlière P

更新日期：1985-05-01 00:00:00

abstract：:OURCIN is a tool to build expert systems, which was developed by a joint team from INRIA and SEMA. We emphasized developing on this system the ergonomic features which make Expert Systems a comfortable and interesting approach for knowledge engineering. ...

journal_title：Biochimie

authors： Quinqueton J

更新日期：1985-05-01 00:00:00

abstract：:The adaptive response appears in E. coli after exposure to low levels of alkylating agents. This system is under the positive control of the ada gene. At least two enzymes are induced during the response: 3-methyladenine DNA glycosylase II and O6-methylguanine DNA methyltransferase. The latter is also the product of t...

journal_title：Biochimie

authors： Defais M

更新日期：1985-03-01 00:00:00

abstract：:Pseudomonas cepacia 122 can grow on several lignin model compounds including the arylglycerol-beta-aryl ethers guaiacylglycerol-beta-coniferyl ether and guaiacylglycerol-beta-guaiacyl ether. Non-phenolic lignin model compounds are not degraded by this bacterium. The enzyme system catalyzing guaiacylglycerol-beta-guaia...

journal_title：Biochimie

authors： Odier E,Rolando C

更新日期：1985-02-01 00:00:00

abstract：:The function of glycerophosphate and malate-aspartate shuttles during glucose metabolism in two strains of Ehrlich ascites tumor cells was evaluated by several experimental approaches. The activities of the enzymes involved in these shuttle systems were assayed in the cytosolic and mitochondrial compartments after cel...

journal_title：Biochimie

authors： Sánchez-Jiménez F,Martínez P,Núñez de Castro I,Olavarría JS

更新日期：1985-02-01 00:00:00

abstract：:The periplasmic histidine permease of Salmonella typhimurium is composed of a soluble histidine-binding protein and three membrane-bound components. These latter are produced in very small amounts and only two, the Q and the P protein, have been previously identified. This paper describes the construction of a plasmid...

journal_title：Biochimie

authors： Ames GF,Nikaido K,Hobson A,Malcolm B

更新日期：1985-01-01 00:00:00

abstract：:This paper reviews recent data on the adenylate cyclase system of the yeast Saccharomyces cerevisiae. Since the discovery of yeast adenylate cyclase mutants and the possibility of molecular biological analysis, adenylate cyclase and the subsequent steps in the cAMP cascade have become subject of intense investigation....

journal_title：Biochimie

authors： Jacquet M,Camonis J

更新日期：1985-01-01 00:00:00

abstract：:We describe a method to compare two fluorescence anisotropy decay curves. After numerical deconvolution of both decays by a non a priori method [1], their difference, D, is considered. The variance is computed for each point of D. A confidence interval is defined which allows a decision to be made as to the significan...

journal_title：Biochimie

authors： Genest D,Privat JP

更新日期：1984-09-01 00:00:00

abstract：:Lysosomal cysteine proteinases were fractionated from partially purified rat muscle lysosomes. By gel filtration on Sephadex G75, cathepsin D was separated from two thiol-requiring proteolytic fractions of Mr 25 000 and 55 000, respectively. By chromatofocusing, the first fraction (Mr = 25 000) was resolved into three...

journal_title：Biochimie

authors： Obled A,Ouali A,Valin C

更新日期：1984-09-01 00:00:00

abstract：:Triiodothyronine (T3), injected daily into rats, induces heart hypertrophy. In a recent work we have shown that a single T3 injection stimulates RNA synthesis and modifies the translational products of myocardial mRNAs in reticulocyte lysates, quantitatively and qualitatively. In this work we show that T3 induces smal...

journal_title：Biochimie

authors： Raymondjean M,Leibovitch SA,Leibovitch MP,Harel J,Kruh J

更新日期：1984-09-01 00:00:00

abstract：:Kinetic properties of PGM1 and PGM2 phosphoglucomutase "primary" isoenzymes from human erythrocytes were studied. The two enzyme forms share a "ping-pong" kinetic mechanism and show similar Km for substrate (glucose 1-P) and cofactor (glucose 1,6-P2). Micromolar concentrations of fructose 1,6-P2 and glycerate 2,3-P2 i...

journal_title：Biochimie

authors： Ninfali P,Accorsi A,Palma F,Fazi A,Piatti E,Chiarantini L,Fornaini G

更新日期：1984-09-01 00:00:00

abstract：:In relation to decreased metabolic sensitivity to catecholamines observed, in vitro, in brown fat of cold-acclimated rats, beta-adrenergic receptors were studied in isolated cells and in a crude membrane preparation from rat interscapular brown adipose tissue. [3H] dihydroalprenolol binding had the same characteristic...

journal_title：Biochimie

authors： Senault C,Le Comte V,Portet R

更新日期：1984-07-01 00:00:00

abstract：:The combination of cDNA and RNA sequencing techniques has enabled determination of the complete sequence of one of the mRNAs coding for the precursor of the small subunit of ribulose bisphosphate carboxylase of Nicotiana sylvestris. In this 898-nucleotide-long mRNA, 540 nucleotides code for the entire 180-amino-acid-l...

journal_title：Biochimie

authors： Pinck M,Guilley E,Durr A,Hoff M,Pinck L,Fleck J

更新日期：1984-07-01 00:00:00

abstract：:The polysaccharide from Aloe vahombe (lilaceae) was submitted to partial hydrolyses by sulfuric, oxalic and phosphoric acid. Some oligosaccharides were isolated and investigated by chemical and 13C NMR spectroscopic methods. Their structure was determined. The results prove unambiguously that in the analyzed material ...

journal_title：Biochimie

authors： Radjabi-Nassab F,Ramiliarison C,Monneret C,Vilkas E

更新日期：1984-07-01 00:00:00

abstract：:With the relation between chemical structure and pharmacological activity as a guide, we have been for some time synthetizing a wide range of beta-amino-ketone derivatives. One of them, 2-(4-methyl-1-piperazinylmethyl) acrylophenone, MPMAP, possesses antimicrotubular activities. This product inhibits 50% of the microt...

journal_title：Biochimie

authors： Mallevais ML,Delacourte A,Lesieur I,Lesieur D,Cazin M,Brunet C,Luyckx M

更新日期：1984-06-01 00:00:00

abstract：:Present knowledge of the different enzymatic steps of the denitrification chains in various bacteria, particularly Paracoccus denitrificans and Pseudomonas aeruginosa has been briefly reviewed. The question whether nitric oxide (NO), nitrous oxide (N2O) and other nitrogen derivatives are obligatory intermediates has b...

journal_title：Biochimie

authors： Henry Y,Bessières P

更新日期：1984-04-01 00:00:00

abstract：:The cellular concentration of cyclic nucleotides is largely dependent upon the activity of the enzymatic system responsible for their degradation: cyclic nucleotide phosphodiesterase. This enzymatic system thus plays a crucial role in the regulation of the multiple functions which are modulated by cyclic nucleotides i...

journal_title：Biochimie

authors： Nemoz G,Prigent AF

更新日期：1984-02-01 00:00:00

abstract：:The activation of the antitumor protein antibiotic neocarzinostatin (NCS) by the carboxyl radical CO-2, a one-electron donor obtained selectively from gamma-ray irradiation of nitrous oxide-saturated formate buffer, has been investigated in the presence and in the absence of DNA at pH 4.7 and pH 7.0. The reaction of N...

journal_title：Biochimie

authors： Favaudon V

更新日期：1983-11-01 00:00:00

abstract：:The synthesis of S100 protein increases toward the end of the exponential phase of growth of clonal rat glial cells C6 in monolayer culture. Moreover the synthesis of this protein can be increased by treatment of C6 cells with the lectin succinylated concanavalin A (succinyl ConA). In order to study the relationship b...

journal_title：Biochimie

authors： Marks A,Law J,Mahony J

更新日期：1983-11-01 00:00:00

abstract：:The effect of bis-(p-nitrophenyl)phosphate on various glycosyltransferases involved in protein glycosylation (sialyl-, fucosyl-, galactosyl-, mannosyl- and glucosyltransferases) have been studied using crude enzyme preparations solubilized from rat spleen lymphocytes. Bis-(p-nitrophenyl)phosphate appears as a common i...

journal_title：Biochimie

authors： Guette F,Cacan R,Montreuil J,Verbert A

更新日期：1983-10-01 00:00:00

abstract：:Complexes of purified 40S ribosomal subunits and initiation factor 3 from rabbit reticulocytes were crosslinked using the reversible protein crosslinking reagent, 2-iminothiolane, under conditions shown previously to lead to the formation of dimers between 40S proteins but not higher multimers. The activity of both th...

journal_title：Biochimie

authors： Tolan DR,Hershey JW,Traut RT

更新日期：1983-07-01 00:00:00

abstract：:A Binding Protein (BP) of nearly 95,000 daltons interacts with the acid phosphatase at the permissive temperature, elevating its molecular weight from 48,000 +/- 2,000 to 136,000 +/- 7,000 in CL and CHR strains of Physarum polycephalum plasmodia. The same molecule has not been observed in the extracts of M3C VIII and ...

journal_title：Biochimie

authors： Demirtas H

更新日期：1983-06-01 00:00:00

abstract：:Viral expression was analyzed in ten cell clones of a Friend erythroleukemia cell line (HFL/b cell line [3]), which had lost its capacity to produce infectious particles. All the ten subclones were non producers but expressed spleen focus forming virus (SFFV) polypeptides in the form of p48-p50gag and gp50-gp52env. On...

journal_title：Biochimie

authors： Moreau-Gachelin F,Robert-Lezenes J,Mathieu-Mahul D,Gisselbrecht S,Larsen CJ

更新日期：1983-04-01 00:00:00

abstract：:After exhaustive pronase digestion, purification by gel filtration and affinity chromatography on concanavalin A, three glycopeptide fractions were obtained from rat hemopexin. Two fractions (I and II) were concanavalin A non-reactive and one (III) was concanavalin A reactive. On the basis of carbohydrate composition,...

journal_title：Biochimie

authors： Bernard N,Lombart C,Strecker G,Montreuil J,Van Halbeek H,Vliegenthart JF

更新日期：1983-03-01 00:00:00

abstract：:Injection of 3,5,3' L-triiodothyronine (15 micrograms/100 g) induces a biphasic enhancement of rat heart ornithine decarboxylase (EC. 4.1.17) activity after 4 and 21 hours. This induction is observed after each daily injection, but to a lesser extent. The properties of partially purified basal enzyme and induced enzym...

journal_title：Biochimie

authors： Raymondjean M,Kneip B,Bachner L,Bogdanovsky-Sequeval D,Schapira G

更新日期：1982-11-01 00:00:00

abstract：:In the process of L-phenylalanine oxidation by Proteus mirabilis cytoplasmic membrane, hydrogen peroxide was produced at a rate corresponding to 1-3 per cent of the total electron flow (30-110 nmoles min-1mg-1). Peroxide was estimated using a fluorimetric assay with horseradish peroxidase, or by anodic oxidation on a ...

journal_title：Biochimie

authors： Sauret-Ignazi G,Laboure-Rossat AM,Jouve HM,Pelmont J

更新日期：1982-10-01 00:00:00

abstract：:In this work, were studied the conditions of erucic acid (cis-docosenoic, n-9) shortening by using Rat liver mitochondrial preparations which were incubated in vitro with [14-14C] erucic acid (22:1), with inhibitors of the respiratory chain (rotenone, cyanide) or not, with activators of either the shortening reaction ...

journal_title：Biochimie

authors： Clouet P,Henninger C,Bezard J

更新日期：1982-10-01 00:00:00

abstract：:The SOS response can be induced indirectly in Escherichia coli by infection with UV irradiated bacteriophage P1, lambda or M13. Induction, monitored quantitatively by means of a sfiA::lac operon fusion, was stronger with the plasmid phage P1 than with lambda, but the kinetics were similar, showing that plasmid and non...

journal_title：Biochimie

authors： D'Ari R,Huisman O

更新日期：1982-08-01 00:00:00

abstract：:Disappearance of 7-ethylguanine and O6-ethylguanine lesions from nuclear DNA is observed on incubation of isolated rat liver nuclei previously treated with ethylnitrosourea. Free 7-ethylguanine but no free O6-ethylguanine is released in the medium. Incubation of methylated or ethylated DNA with chromatin proteins lead...

journal_title：Biochimie

authors： Renard A,Lemaitre M,Verly WG

更新日期：1982-08-01 00:00:00

abstract：:Treatment of wild type Escherichia coli with cis -Pt(NH3)2Cl2 increased the survival and frequency of clear plaques formation of lambda phage damaged by UV radiation. The reactivation process was present in an uvrA mutant and abolished in a lexA host. Trans-Pt(NH3)2Cl2 and [Pt(dien) Cl]Cl (dien = 2HN-CH2-CH2NH-CH2-CH2...

journal_title：Biochimie

authors： Alazard RJ,Germanier M

更新日期：1982-08-01 00:00:00

abstract：:A discriminating system capable of recognizing the oxygenated sterols was investigated in human lymphocytes. After labelling entire cells with 25-hydroxy [3H] cholesterol (10 nM) the cytosol was ultracentrifuged on a linear sucrose density gradient. Bound 25-hydroxy [3H] cholesterol was located in a single peak with a...

journal_title：Biochimie

authors： Defay RE,Astruc ME,Roussillon S,Descomps B,Crastes De Paulet A

更新日期：1982-05-01 00:00:00

abstract：:The solubilizing powers of taurocholate, taurochenodeoxycholate and tauroursodeoxycholate for monoolein and cholesterol, and the size of the bile salt-monoolein-cholesterol micelles have been determined. For the three bile salt species, the micellar size depends on the saturation with monoolein. As a result, for a giv...

journal_title：Biochimie

authors： Montet JC,Lindheimer M,Reynier MO,Crotte C,Bontemps R,Gerolami A

更新日期：1982-04-01 00:00:00

abstract：:Attempts at isolating individual human milk proteins showed that cross interactions made it difficult to obtain of homogeneous components. A new method was devised, based on complete precipitation of milk proteins with saturated ammonium sulphate and progressive solubilization of the precipitate on a column of Sephade...

journal_title：Biochimie

authors： Brignon G,Ribadeau-Dumas B

更新日期：1982-03-01 00:00:00

abstract：:Adipose tissue lipoprotein lipase activity (LPL) falls dramatically during experimental infection of mice by Plasmodium chabaudi. The decrease in this activity accounts for the seric accumulation of triacyl-glyceride-rich lipoproteins (chylomicrons mainly and Very Low Density Lipoproteins (VLDL) partly). This loss of ...

journal_title：Biochimie

authors： Al Rifai W,Maurois P,Fruchart JC,Charet P

更新日期：1982-02-01 00:00:00

abstract：:Synthetic antiestrogens such as Tamoxifen (Nolvadex) are currently used to treat postmenopausal breast cancer. Their mechanism of action is reviewed at the cellular and molecular level, through data of the literature and the current view of the author. Synthetic antiestrogens are mostly acting directly on breast cance...

journal_title：Biochimie

authors： Rochefort H

更新日期：1982-02-01 00:00:00

abstract：:Theoretical and experimental investigations on the conformations of synthetic polytripeptides, with Gly-R2-R3 general repetitive sequence, are of great interest for the comprehension of the factors which contribute to the triple helix stabilization. Using appropriate molecules as models, we have investigated by the PC...

journal_title：Biochimie

authors： Cabrol D,Broch H,Vasilescu D

更新日期：1981-11-01 00:00:00

abstract：:The proton NMR data of a synthetic model of deoxy-myoglobin and hemoglobin show one that:(i) the ground state of the high spin iron (II) complex must be described by at least two electronic levels separated by ca.200 cm-1 and resulting from the removal of the degeneracy of the 5E level; (ii) this in-plane rhombic dist...

journal_title：Biochimie

authors： Mispelter J,Momenteau M,Lhoste JM

更新日期：1981-11-01 00:00:00

abstract：:The lipoprotein complex from human gallbladder bile was challenged with anti-IgA and anti-IgG antisera in order to determine whether the apolipoprotein complex isolated from the detergent-free form of bile lipoprotein contains IgA and IgG. The apolipoprotein complex indeed crossreacts with anti-IgA and anti-IgG. In ad...

journal_title：Biochimie

authors： Vigne JL,Nalbone G,Lafont H,Lairon D,Chabert C,Domingo N,Hauton JC

更新日期：1981-10-01 00:00:00